Phytochemical and anti-microbial investigations on Oroxylum indicum
Author (s): Padma Charan Behera, Sourav Khawas, Arya Ghosh, Manik Ghosh, Prasanta Kumar Choudhury
Department of Pharmaceutical Sciences, Jharkhand Rai University, Kamre, Ranchi, Jharkhand,
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra,
Ranchi, Jharkhand, Pin-835215
Royal College of Pharmacy and Health Sciences, Berhampur, Odisha, Pin-760002
Oroxylum indicum also known as ‘Sonapatha’ is an important herb in Ayurvedic medicine and indigenous medical system for over thousands of years. Oroxylum indicum, Sonapatha is traditionally used to treat asthma, biliousness, bronchitis, diarrhoea, dysentery, fevers, vomiting, inflammation, leukoderma, skin diseases, rheumatoid arthritis, wound injury, and deworm intestine. Oroxylum indicum has been collected from the premises of IMMT(Formerly known as RRL,Bhubaneswar and authenticated by Dr N.K.Dhal,Senior principal scientist,IMMT,Bhubaneswar.The extraction process for Oroxylum indicum has been done by using solvent of gradient polarity. The extractive value was in order of water> ethanol> chloroform> benzene pet.ether. Column chromatographic techniques for different extract by using gradient elution process has been followed and the eluents are being subjected for antimicrobial activity by using different microbes such as Acineovacter, E.Coli, S.aureus, M.luteus and R.palanticola. All the bacterial strains were obtained from microbial type culture collection and gene-bank, International depository authority, IMTECH (Chandigarh), India maintained in nutrient broth at -20˚C. All the extracts were subjected for the presence of different type of secondary metabolites such as alkaloids, flavonoids, tannins, anthraquinones, saponins, carbohydrates, sugar, steroid, glycoside, amino acid.
Keywords: Celecoxib, BetaCyclodextrin, kneading, in situ ophthalmic gel, In vitro drug release