Quality Standardization and Characterization of Rutin Fraction and Protein Estimation with Antihelminthic Property from the Methanolic Plant Extract of Olax Psittacorum.
Author: Sidhanta Behera1, Deviprasad Patra2, Lopamudra Adhikari2
Abstract: From ancient times, herbal plants are used for the treatment of various diseases due to its minimal or no side effect. The present study aims at isolation of flavonoid, rutin from the methanolic leaf extract of Olax psittacorum. Protein estimation and anti-helminthic activity of the methanolic plant extract was carried out. Fractionation was carried out using column chromatography method as the separation technique. The fractions were analyzed and compared by TLC (thin layer chromatography) using the standard. The solvent system selected was ethyl acetate: chloroform: butanol (4:2:4). The Rf value on TLC was observed 0.52. Antioxidant radical scavenging assay like DPPH (2,2- diphenyl-1-picrylhydrazyl) radical scavenging assay, ABTS (2,2’- azino-bis(3-ethylbenzothiazoline-6- sulfonic acid)diammonium salt) radical scavenging assay, Hydrogen peroxide (H2O2) assay, by taking ascorbic acid as standard. The regression value of standard was observed 0.938, 0.939 and 0.961 for DPPH, ABTS.
Keywords: Olax Psittacorum, Rutin, Antimicrobial activity, Antioxidant activity