Article 5

Research Article

A RP-HPLC Method Development and Validation for the Estimation of Atorvastatin in Bulk and Pharmaceutical Dosage Forms

Author (s): Dasari Sailesh1, Saroj Kumar Raul1*, Gopal Krishna Padhy2


Abstract:

An easy RP-HPLC method was developed and validated considering the parameters like selectivity, linearity, precision and accuracy for the rapid assay of atorvastatin in tablet dosage form. Flow rate of 1 mL/ min was engaged on an Eclipse XDB plus column (C18) at a temperature of 40 .C. Mobile phase was comprised of Acetonitrile: Potassium dihydrogen orthophosphate buffer at a ratio of 70:30 (v/v) and detection wavelength was chosen at 232nm. Linearity was detected in between the concentration range of 5-60 μg/ mL Retention time of atorvastatin was observed at 2.273 min. ICH guidelines was followed for method validation. The projected process can be effectively useful for the approximation of atorvastatin in therapeutic dosage forms.


Keywords:

RP-HPLC, Method development, ICH guidelines, Validation, Atorvastatin.