FORMULATION AND IN-VITRO CHARACTERIZATION OF LAMIVUDINE EMBEDDED NATURAL RESIN MICROSPHERES USING OLIBANUM AS DRUG CARRIERS
Author (s): Satyajit Panda*, Ashish Sahu, Biswa R. Mohanty, Bhabani S. Nayak, Bibaswan Mishra
Abstract: The present study was aimed to formulate and characterize controlled release microspheres of lamivudine using a natural resin olibanum as novel microencapsulating carrier. An o/o emulsification-solvent evaporation method was employed for design of different batches which can be made industrially feasible. The batches designed were characterized in-vitro for formulation yield, encapsulating efficiency, particle morphology, flow properties, infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), release behavior, release kinetics etc. The drug loaded resin microspheres were resulted to be discrete, free flowing and roughly sphere-shaped. Microencapsulation efficiency revealed in narrow and even distribution of lamivudine among various formulated batches. XRD and DSC reports resulted a minor change in lamivudine physical state. Release of lamivudine from optimized batches was slow with release period of over 24 hours. Release was found to be dependent on ratio of core to coat in the formulations. The mechanism drug release was detected to be following Fick’s law. Various processing parameters like as core to coat ratio, stirring speed, concentration of surfactant, processing medium volume, etc. for the process of emulsification was identified in relation with the microencapsulation efficiency and release behaviors of drug. The drug loaded resin microspheres revealed release characteristics in a controlled manner and established to be ideal for once a day dosing.
Keywords: Controlled release, Lamivudine, Microspheres, Olibanum, Resin