DEVELOPMENT AND VALIDATION OF A LIQUID CHROMATOGRAPHIC METHOD FOR THE ESTIMATION OF A MYORELAXANT DRUG IN PHARMACEUTICAL DOSAGE FORMS
Author (s): Sasmita Kumari Acharjya*, Smaranika Pradhan, Partha Sarathi Das
Abstract: The main objective of this study is to develop and validate a liquid chromatographic method for the estimation of a myorelaxant drug i.e. Thiocolchicoside in various marketed pharmaceutical formulations. In this method reverse phase liquid chromatograph having ODS C18 column (250×4.6mm packed with 5µ), methanol and 10 mM TBAHS (tetra butyl ammonium hydrogen sulfate) as mobile phase in isocratic mode at a flow rate of 0.8 ml/min is used to achieve chromatographic separation. Benzoic acid was employed as an internal standard (IS). The chromatograms were recorded at 259nm. The drug obeys linearity in the concentration range of 2.5–400µg/ml with correlation coefficient 0.999. Retention time of drug and internal standard was found to be 4.162 ± 0.004min and 8.447 ± 0.016min respectively.
Keywords: Thiocolchicoside, Liquid chromatography, TBAHS, Myorelaxant