SIMULTANEOUS ESTIMATION OF SACUBITRIL AND VALSARTAN IN BULK AND PHARMACEUTICAL DOSAGE FORM BY USING RP-HPLC
Author (s): Kirtimaya Mishra1*, K. Aditya Prasanna2, Snigdha Rani Behera2
Abstract: A new and simpler method was developed with the Inertsil ODS 3V C18 column, linked to a C18 guard cartridge and PDA detection at 239 nm, to quantitably detect sacubitril (SAC) and valsartan (VAL). The optimized mobile phase acetonitrile (ACN), triethylamine buffer (TEA) was pumped at 0.7 mL/min. The method was linear between 5-15 µg/mL for SAC and 2.5-7.5 µg/mL for VAL, statistically validated for its accuracy, precision, and linearity. The additives in the commercial tablet were found to have no interference in the method. The currently developed method for estimating compounds SAC and VAL associated with the tablet dosage form can be used routinely.
Keywords: Sacubitril, Valsartan, Simultaneous estimation, RP-HPLC.