ANGIOGENIC TOXICITY STUDY AND CHROMATOGRAPHIC ESTIMATION FOR METHANOLIC EXTRACT OF HELICONIA PSITTACORUM
Author (s): Smriti Roy, Lopamudra Adhikari
Abstract: Ethnobotanically, Heliconia psittacorum is used for the treatment of ulcers in the scalp in South America. This study aims at standardisation and quality evaluation of the isolated quercetin from the leaf methanolic extract (LME) of H.psittacorum. Methanolic extracts of dried leaves were prepared and was subjected to phytoconstituents screening. The extract was then subjected to different chromatographic techniques such as thin layer chromatography (TLC), column chromatography and High Performance Liquid Chromatography (HPLC) for quality evaluation of the isolated quercetin. The extract was also subjected to antioxidant assay and anti-bacterial assay and also determination of anti-angiogenic activity of the extract using Chorioallantoic membrane assay (CAM). The method proved to be accurate, sensitive, and precise with the recovery values within the acceptable ranges. The extract showed good inhibition towards E. coli and showed acceptable result in the antioxidant activity showing the IC50 value at minimal concentration. On subjecting the CAM to different concentrations of LME the average score determined showed strong anti-angiogenic activity at 100-150µg/ml. So LME can be used for further investigation for its different medicinal values.
Keywords: Heliconia psittacorum, standardisation, quality evaluation, HPLC, quercetin, antioxidant, antimicrobial, chorioallantoic membrane.