Article 5

RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ASPIRIN AND OMEPRAZOLE IN BULK AND TABLET DOSAGEFORM

Author (s): V. Anil Kumar1, Soraj Kumar Raul1, Gopal Krishna Padhy2*


Abstract: A reverse phase HPLC method was successfully developed and validated for concurrent assessment of omeprazole and aspirin for bulk and pharmaceutical formulation. The method was developed employing  a reversed phase C18 column. A optimized mobile phase having a composition of acetonitrile-methanol (20:80 v/v) was used. The experiment was carried out at flow rate of  0.6 ml/min and wavelength of  233 nm.  Retention times for omeprazole and aspirin were found at 2.667 and 1.720 min respectively. Across a concentration span of 10-50 μg/ml and 5-25 μg/ml the method was linear for aspirin and omeprazole, respectively. The proposed method have potential for the regular quality scrutiny of this combination in bulk and pharmaceutical dosage form.


Keywords: RP-HPLC, Aspirin, Omeprazole, ICH, Simultaneous estimation.